About Next-Gen Sequencing

The first-generation Sanger sequencing method has inherent technical limitations including low-throughput, slow speed, high cost, and difficulty to analyze allele frequency. To achieve the goal of $1,000 human genome, new sequencing technologies have been coming out since 2004. These technologies are massively parallel, thereby achieving high-throughput. The streamlined sample prep step prior to sequencing leads to significant savings in time and cost. Since each sequencing reaction is carried out on one piece of DNA, different alleles can be analyzed at the same time.

 

Key steps of the sequencing-by-synthesis-based Illumina sequencing procedure


CMADP Events

Ralph N. Adams Lectureship
KU Chemistry Dept.

Robert Kennedy, Ph.D.
Hobart H. Willard Distinguished University Professor and Chair of Chemistry
Professor of Pharmacology
University of Michigan

"The Nanoliter Lab: Droplet Microfluidics for Screening and Sensing"
Friday, September 6, 2019 at 4:00pm
Integrated Science Building, Room 1154

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