Services and Pricing

Click to read more about specific areas of GSC pricing

NextSeq 550 & MiSeq Pricing

Library Prep & Equipment Fees

Click to email the GSC to request services or to view the current sequencing queue

Service Request Form

Current Queue
 

Users can download the Illumina Sequencing Coverage Calculator to estimate the number of reads required for your experiment.

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GSC Sample Submission Guidelines

Click here to download the RNA/DNA Submission Form.

Click here to download the Library Submission Form.

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gDNA for DNAseq Library Construction

• We recommend that gDNA be submitted at a concentration of 20 ng/µL in 60 µL total
  • 5 µL will be used for sample QC, 55 µL will be used for library prep  ​
• gDNA should be RNase treated prior to submission and the quality verified by electrophoresis.  
• We recommend that you quantify gDNA with Qubit
• DNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.
• Sample names must include "legal" characters please use alpha numeric characters and "-" or "_" - dashes or underscores
  • Please do not include " . ) ( / \ } { ] [ ? = + > < : ; " ' * ^ | & " - periods, parentheses, back or forward slashes, brackets, question mark, equals sign, addition sign, chevrons, colon, semicolon, quote sign, apostrophe, asterisk, carrot, bar or ampersand.

Total RNA for RNAseq Library Construction

• We recommend that total RNA be submitted at a concentration of 20 ng/µL in 60 µL total 
  • ​​10 µL will be used for sample QC, 50 µL will be used for library prep 
• We recommend that total RNA be Trizol extracted and treated with DNase prior to submission
• We recommend that you quantify RNA with Qubit
• We strongly recommend RNA to be stored on dry ice or at -80°C until submission.
• RNA should be submitted in clearly labeled 1.7 mL tubes, please limit sample names to 8 characters.
• Sample names must use alphanumeric characters and "-" or "_" - dashes or underscores
  • Please do not include " . ) ( / \ } { ] [ ? = + > < : ; " ' * ^ | & " - periods, parentheses, back or forward slashes, brackets, question mark, equal sign, addition sign, chevrons, colon, semicolon, quote sign, apostrophe, asterisk, carrot, bar or ampersand.

Investigator Prepared Libraries

• Libraries must be prepared according to standard commercially available library kits (eg. Illumina TruSeq, Nextera, NEBNext, etc).
• Please contact the GSC if you plan to submit custom libraries.
• Libraries are submitted as 30 µL aliquots at a concentration of ~10 nM.
  • To dilute samples to 10 nM, use nM conversion calculator, this will give an estimated nM concentration
  • Use Qubit quantification to get ng/µL concentration and TapeStation/Bioanalyzer gel analysis for average bp size estimation
• Accurate quantification of libraries is essential for flow cell clustering. We strongly recommend that libraries are quantified by qPCR. The GSC can perform qPCR of a sample for a fee.
• Please submit these samples in a clearly labeled 1.7 mL tube; please limit the sample names to 8 characters.
• Sample names must use alphanumeric characters and "-" or "_" - dashes or underscores
  • Please do not include " . ) ( / \ } { ] [ ? = + > < : ; " ' * ^ | & " - periods, parentheses, back or forward slashes, brackets, question mark, equal sign, addition sign, chevrons, colon, semicolon, quote sign, apostrophe, asterisk, carrot, bar or ampersand.

Sample Drop Off

Samples can be delivered to the GSC Monday through Friday, between 9:00 AM and 5:00 PM.

Samples can also be mailed to the GSC on dry ice at this address:

          Genome Sequencing Core

          University of Kansas

          1030 Haworth Hall

          1200 Sunnyside Avenue

          Lawrence KS 66045